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Verlag GmbH
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Sato Pharmaceutical Co Ltd
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Epigenomics ag
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Image Search Results
Journal: JCI Insight
Article Title: Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells
doi: 10.1172/jci.insight.158457
Figure Lengend Snippet: ( A ) Diagram of the hypothesis of PBA-derived phenylacetyl-CoA competing with acetyl-CoA to interfere with SAT1-mediated polyamine catabolism and acetyl-CoA consumption. SPDSY, spermidine synthase; PAO: peroxisomal N 1 -acetyl-spermine/spermidine oxidase; SMOX, spermine oxidase. ( B ) Acetyl-CoA level in HEK293T cells with SAT1 overexpression with or without 2 mM PBA treatment. Cells from 4 separate experiments were stored at –80°C and then tested in a single plate. n = 4; 1-way ANOVA multiple comparisons (matched). ( C ) Dot blot of acetyl-lysine in HEK293T cells with SAT1 overexpression with or without 2 mM PBA treatment. The values of the quantification were normalized with the control samples. ( D ) Western blot of the samples in C . Acetylated histones and nonhistone proteins in the bracket areas were quantified separately and normalized with β-Actin level. All values were further normalized by the control samples. ( E ) Western blot of HA-tagged SAT1 overexpressed in HEK293T cells with PBA, MG132, or the combination treatment. The HA-SAT1 level was normalized with the β-Actin level. The values of the cells without HA-SAT1 plasmid transfection were set as background. All the values were further normalized by that of the cells with indicated treatment. ( F ) Western blot of SAT1 induced by DENSPM in patient fibroblasts (CMS1849a) with indicated treatment. The SAT1 level was normalized with the β-Actin level. Values of the cells without DENSPM treatment were set as background. All values were further normalized by that of cells with DENSPM and without PBA or MG132 treatment. Images in C – F are representative of 3 separate experiments. n =3; * P < 0.05, ** P < 0.01, *** P < 0.001; 1-way ANOVA multiple comparisons (matched). Data represent mean ± SEM.
Article Snippet: The
Techniques: Derivative Assay, Over Expression, Dot Blot, Control, Western Blot, Plasmid Preparation, Transfection
Journal: International Journal of Molecular Sciences
Article Title: Bioactive Carbohydrates and Peptides in Foods: An Overview of Sources, Downstream Processing Steps and Associated Bioactivities
doi: 10.3390/ijms160922485
Figure Lengend Snippet: Bioactive peptides derived from food sources and their use as bioactives in commercial products.
Article Snippet: VY , Antihypertensive ,
Techniques: Derivative Assay, Sequencing, Capsules, Control, Cream